Phosphothreonine anticorps (Biotin)

N° du produit ABIN5596877

N° du produit (Fournisseur): 600-406-263

Cet anticorps Lapin Polyclonal détecte spécifiquement Phosphothreonine dans WB, IP, ELISA et IHC. Il présente une réactivité envers .

Aperçu rapide pour Phosphothreonine anticorps (Biotin) (ABIN5596877)

Antigène: Phosphothreonine

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Phosphothreonine est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-Phosphothreonine anticorps (Biotin)

N° du produit (Fournisseur): 600-406-263

Fournisseur: Rockland

Fonction: Phosphothreonine Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.

Attributs du produit: Synonyms: PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit

Purification: Anti-PhosphoThreonine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to biotin and subsequent purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: PhosphoThreonine affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: PhosphoThreonine affinity-purified antibody is tested for use in ELISA and western blotting assays.  The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues.  The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store anti-pT antibody at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Phosphothreonine

Antigène: Phosphothreonine

Classe de substances: Amino Acid

Sujet: Background: Phospho-Threonine Antibody detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.